| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156122 |
| Chromosome: | chromosome 17 |
| Location: | 1300443 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705700 | PROB1,GGK1 | Glutamate 5-kinase; (1 of 3) 2.7.2.11 - Glutamate 5-kinase / Gamma-glutamyl kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCTCCACCCTCATGATGCCCGCAGCC |
| Internal bar code: | CCGCCCCATATGTTTCTCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACGGGTGAGAAAGGAGG |
| Suggested primer 2: | TACCACCACCTCCAGTGTGA |