Insertion junction: LMJ.RY0402.156168_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.156168
Chromosome:chromosome 2
Location:740376
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g078500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGAAAAGGACGGGCAACCGACCCATCCC
Internal bar code:TGCGCCTGTATTTGCGTAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:603
LEAP-Seq percent confirming:98.5021
LEAP-Seq n confirming:1644
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCAAGAGTCGAAGTTGCC
Suggested primer 2:GTGGTTAGAATCTTGCGGGA