| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156204 |
| Chromosome: | chromosome 16 |
| Location: | 5357808 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681750 | FAP381 | Flagellar Associated Protein 381; (1 of 5) K01537 - Ca2+-transporting ATPase (E3.6.3.8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCTCCTGGACCCACCTTGCCCTTGCCC |
| Internal bar code: | GGAGTTTCAATCTGTGCAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1082 |
| LEAP-Seq percent confirming: | 99.6774 |
| LEAP-Seq n confirming: | 927 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTGCTTCCTAACTTGC |
| Suggested primer 2: | GGAACTTGCGGATGTTCTGT |