Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.156207 |
Chromosome: | chromosome_11 |
Location: | 2073188 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g468850 | FAP152 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAATTCACTGCAGGACAGAGGAGAGCGAT |
Internal bar code: | AACGCGCTGTAGGAGCCAGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 968 |
LEAP-Seq percent confirming: | 97.4839 |
LEAP-Seq n confirming: | 1821 |
LEAP-Seq n nonconfirming: | 47 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCTTTTGGATCTGTGGGA |
Suggested primer 2: | GTTGAACAAGATGGGCAGGT |