| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156228 |
| Chromosome: | chromosome 7 |
| Location: | 1623649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325101 | (1 of 2) PTHR30336 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCACAGCTGCGTGGGTTTTCAGGCAGG |
| Internal bar code: | ACTTCAGCCGACAAAAGTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 79.3968 |
| LEAP-Seq n confirming: | 1869 |
| LEAP-Seq n nonconfirming: | 485 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGCATGACACGCTCTT |
| Suggested primer 2: | ATGCGTGTCAAAGGTCATCA |