| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156228 |
| Chromosome: | chromosome 7 |
| Location: | 1624031 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325150 | TGL12 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase; Putative triacylglycerol lipase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGATCAGCCGGTGACAGCTCCTATTT |
| Internal bar code: | GGATAAGCGGTTCTCTCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 98.6726 |
| LEAP-Seq n confirming: | 2230 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGCATACCCCAGCACAA |
| Suggested primer 2: | ATATCGCAGCGTGCCTTACT |