| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156228 |
| Chromosome: | chromosome 13 |
| Location: | 5027397 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606800 | (1 of 2) PTHR30336//PTHR30336:SF4 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCTGGAAACCCTCGGTCTGCGTTCCTC |
| Internal bar code: | CAGACGCACGTAATGCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 99.2736 |
| LEAP-Seq n confirming: | 410 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTCCAATGGGTGTTTG |
| Suggested primer 2: | AGCGGTGTCAATAAGATGCC |