| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156236 |
| Chromosome: | chromosome 6 |
| Location: | 580690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253200 | PKY1 | Protein tyrosine kinase; (1 of 1) PTHR23257:SF534 - SERINE/THREONINE-PROTEIN KINASE ROCO7-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCACCCAGACATGGGGTTGTGTGTACGA |
| Internal bar code: | GTTTTAGGAGAACAATGAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 87 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGAATCCCCGTCTTGT |
| Suggested primer 2: | GTAGCCGCTCCTGATCTGAC |