Insertion junction: LMJ.RY0402.156237_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.156237
Chromosome:chromosome 1
Location:3932145
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g025650 (1 of 1) IPR013286 - Annexin, type VII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCACCCGTCGTGGGCATTTGTCTTCGTG
Internal bar code:TGTGCTGCTTGACTATATTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1154
LEAP-Seq percent confirming:99.5207
LEAP-Seq n confirming:13080
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTCTGTGGCGTTGTTAGT
Suggested primer 2:CTTCGTGACTCCCGTCTAGC