| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156251 |
| Chromosome: | chromosome 12 |
| Location: | 9118748 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542650 | (1 of 1) IPR000210//IPR011333//IPR013017 - BTB/POZ domain // POZ domain // NHL repeat, subgroup | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCGGTTCCAGTCTGCACCTACCCATG |
| Internal bar code: | ATCCTTTGTCACCGTGAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTAACCACCAACCTAT |
| Suggested primer 2: | TCACGGCCTTTCCTTATGTC |