| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156254 |
| Chromosome: | chromosome 13 |
| Location: | 4535983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602750 | (1 of 5) PTHR32467:SF15 - AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGCCCGTCCACACGCTGCTGCCGATC |
| Internal bar code: | GATCGGCGTTGGGCCTCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 54.2926 |
| LEAP-Seq n confirming: | 449 |
| LEAP-Seq n nonconfirming: | 378 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGCTGTTGAGAGACTG |
| Suggested primer 2: | GCAATTTCCTGGAGACTCGT |