| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156258 |
| Chromosome: | chromosome 7 |
| Location: | 5106378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347850 | (1 of 1) PF08123 - Histone methylation protein DOT1 (DOT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCGCCGCACGAGCGTCCGCCCCTCCC |
| Internal bar code: | TGTTTCTACCCGCTCGAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.8326 |
| LEAP-Seq n confirming: | 2982 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCACAAATCAAAACCCT |
| Suggested primer 2: | GGGCGCCTCTCTCTTTCTAT |