Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.156274 |
Chromosome: | chromosome_14 |
Location: | 1869010 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g620600 | PHC2 | Cell wall protein pherophorin-C2 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGCGTTAGTTTGGCGTGCGATGCAATCAT |
Internal bar code: | TGCTGCGAACCAAGGAGGGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 653 |
LEAP-Seq percent confirming: | 99.837 |
LEAP-Seq n confirming: | 1837 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTTGGAGCAGCAGTAATC |
Suggested primer 2: | GAGCATCAGCCACTTGTGAA |