| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156281 |
| Chromosome: | chromosome 10 |
| Location: | 819504 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423400 | (1 of 2) 1.3.1.89 - tRNA-dihydrouridine(47) synthase (NAD(P)(+)) / tRNA-dihydrouridine synthase 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGGCGTGGAAGCGTGGTGTTCAACGC |
| Internal bar code: | ACGGGGGGAGCATCGGAGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 79.6373 |
| LEAP-Seq n confirming: | 2108 |
| LEAP-Seq n nonconfirming: | 539 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCTTGGTTCCGTCCTG |
| Suggested primer 2: | ACCTGTCCCTCTCTTCCTGC |