Insertion junction: LMJ.RY0402.156285_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.156285
Chromosome:chromosome 12
Location:6363537
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g537500 (1 of 1) PTHR11200//PTHR11200:SF136 - INOSITOL 5-PHOSPHATASE // PHOSPHOINOSITIDE PHOSPHATASE SAC1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCACATGCCTGGAACCCCGCTCCGCCC
Internal bar code:GAGGGGTTGGACGTAGGCTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:99.0741
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACTCTCTCTCCCCG
Suggested primer 2:AACAGGTTTATGGCGTCCTG