Insertion junction: LMJ.RY0402.156305_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.156305
Chromosome:chromosome 3
Location:513974
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g145247 TEF11 S-isoprenylcysteine O-methyltransferase; (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACAAACGTGCAGCTACGACTTACCCAG
Internal bar code:TCAACGGCTTGTACGATACACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1040
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:661
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAACGCAAGCGAGTAACCCT
Suggested primer 2:AAAGCCCAACATGTCGAAAC