| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156317 |
| Chromosome: | chromosome 14 |
| Location: | 3563080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGGATCGCTTTCCTTGCTGCAACTTT |
| Internal bar code: | CACGTGGCGGTCTTTGATACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.7718 |
| LEAP-Seq n confirming: | 1749 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCCTTCGCTTCTCT |
| Suggested primer 2: | GGAAAGAGGGTCGGGTAGAG |