| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156329 |
| Chromosome: | chromosome 14 |
| Location: | 2382843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624201 | (1 of 1) PTHR10438//PTHR10438:SF247 - THIOREDOXIN // THIOREDOXIN-LIKE PROTEIN CDSP32, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGTCGCACGCAAACGGAGCGACATGA |
| Internal bar code: | AGAATCTGAAGTTTACGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1163 |
| LEAP-Seq percent confirming: | 99.6643 |
| LEAP-Seq n confirming: | 2969 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACTCGCTGCCTTGTCCT |
| Suggested primer 2: | CTAAACCTCCTCCGGTCCTC |