Insertion junction: LMJ.RY0402.156338_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.156338
Chromosome:chromosome 10
Location:3044377
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g441300 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACGCAAACTTTGCCCTTGATTGATATT
Internal bar code:TTTCGGACACAGTGTCCACCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:99.4226
LEAP-Seq n confirming:861
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGCCGCACTACTTTGA
Suggested primer 2:TAGTGAGCCCGCTATCTCGT