| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156349 |
| Chromosome: | chromosome 2 |
| Location: | 6091616 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113100 | (1 of 1) PTHR14154:SF3 - MITOCHONDRIAL PYRUVATE CARRIER 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGATTCCGCTGGCCTTCTGCCATTGGTC |
| Internal bar code: | AAGAGCCTGTGCCTACAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.7072 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCGGTCCAAAGACAACT |
| Suggested primer 2: | GACCGAGGGTGTTGTGCTAT |