| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156370 |
| Chromosome: | chromosome 2 |
| Location: | 3396593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095104 | (1 of 1) K15193 - protein SPT2 (SPTY2D1, SPT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCATCATTGGTAGGCAGTGGCCTATCG |
| Internal bar code: | TCGCAACGCTTGGATGTGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 99.6262 |
| LEAP-Seq n confirming: | 11194 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATGAAGCGGCTCAAGAA |
| Suggested primer 2: | GAGCACACGGTACGGATTTT |