| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156380 |
| Chromosome: | chromosome 5 |
| Location: | 3384528 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240900 | (1 of 1) IPR000001//IPR024079 - Kringle // Metallopeptidase, catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTGTGCCTGCGGAGGCGCCCGCTGGT |
| Internal bar code: | GCCGGTGAAGGAACTATCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 97.4576 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTAGAGCGTGTGGTGGA |
| Suggested primer 2: | AGATACCTTGGCACAGGTCG |