| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156396 |
| Chromosome: | chromosome 10 |
| Location: | 5544585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459500 | NGB1 | Nucleolar GTP-binding protein; (1 of 1) PTHR11702:SF4 - NUCLEOLAR GTP-BINDING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGATGCCCAAGCACTTGTTCAGCGGGAA |
| Internal bar code: | AATTGCATTGCTGCGCCTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.4096 |
| LEAP-Seq n confirming: | 3031 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | TGATGAGCCTAGCAGTGGTG |