| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156400 |
| Chromosome: | chromosome 14 |
| Location: | 3142880 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628800 | (1 of 1) 3.4.21.112 - Site-1 protease / Subtilisin/kexin isozyme 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAATGTTGTGTGTTTGCTGTGTACCAT |
| Internal bar code: | TTGTATCGAGAGTGGAAAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGGTGGATTGGAACAA |
| Suggested primer 2: | GGCAATTTGCTAGCTTGGAG |