| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156469 |
| Chromosome: | chromosome 10 |
| Location: | 1257250 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426800 | (1 of 1) IPR000104//IPR003480 - Antifreeze protein, type I // Transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGTTAGCAGAGCCAATACCGTATTGTC |
| Internal bar code: | TCGTATCATCCGCCAAGCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 99.6461 |
| LEAP-Seq n confirming: | 3097 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCGGTGCCGTACTGAG |
| Suggested primer 2: | CGCTCAAACTCTCACCATCA |