Insertion junction: LMJ.RY0402.156497_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156497
Chromosome:chromosome 6
Location:8812004
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g310200 DGAT3 Diacylglycerol acyltransferase, Type 3; (1 of 1) PTHR11780//PTHR11780:SF8 - NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTACATGTCACCCTCCTCTGCCCTAAG
Internal bar code:GAACTCGGGGCCAGACAGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:669
LEAP-Seq percent confirming:93.9124
LEAP-Seq n confirming:1265
LEAP-Seq n nonconfirming:82
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACGGGTAGTCACATTCT
Suggested primer 2:GGTCAGATGGAGTTGACGGT