| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156508 |
| Chromosome: | chromosome 16 |
| Location: | 6811932 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCACAGCCCGGCTCCCCTCCCAGAGCC |
| Internal bar code: | GGGACGATTGTGAGGGGGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 77.5225 |
| LEAP-Seq n confirming: | 1552 |
| LEAP-Seq n nonconfirming: | 450 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTGTTGAATTCCGAGT |
| Suggested primer 2: | CGCATCATCAATGGATCAAC |