| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156508 |
| Chromosome: | chromosome 16 |
| Location: | 6811939 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAAATGGTAGCTTACCATCTTGGCCGA |
| Internal bar code: | AGCACACTGCGTTGTCTGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.7462 |
| LEAP-Seq n confirming: | 4716 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTGTTGAATTCCGAGT |
| Suggested primer 2: | CGCATCATCAATGGATCAAC |