| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156517 |
| Chromosome: | chromosome 14 |
| Location: | 3296873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629750 | MME2 | (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase; NADP-dependent malic enzyme 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCAGGCAGACACACCACCCACTGGCAC |
| Internal bar code: | GGATCCACCCGGACGGCAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 85.8639 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGGGTCCTGAATGTTGA |
| Suggested primer 2: | AGAGCGTGTCATGCTGTTTG |