| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156522 |
| Chromosome: | chromosome 10 |
| Location: | 3959417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448550 | (1 of 3) PF14240 - YHYH protein (YHYH) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGAGGCTGGTCGTTACACTTTTGGCA |
| Internal bar code: | ACGAAGATTTCCAGCGTTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 99.5888 |
| LEAP-Seq n confirming: | 1453 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGAGTGCGTAGGACAGA |
| Suggested primer 2: | GCGTTTTTGGGCACTGTTAT |