| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156537 |
| Chromosome: | chromosome 12 |
| Location: | 9231916 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541650 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTAAGACACCCATTCCATGCGCCAAC |
| Internal bar code: | TGAACTAAGTGGCTATATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.5387 |
| LEAP-Seq n confirming: | 1079 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTGGCTACATTCTTAGC |
| Suggested primer 2: | GCGCAACTGCAACATAGAAA |