| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156539 |
| Chromosome: | chromosome 17 |
| Location: | 3261918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722900 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACAGTTGCGGGAGACACCTGCTGACCCG |
| Internal bar code: | GGGGTCGGGTGAACCCAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.1786 |
| LEAP-Seq n confirming: | 483 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTATGCCTAACAACGC |
| Suggested primer 2: | TTAGGAATGGTCTTTGCGCT |