Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.156560 |
Chromosome: | chromosome_14 |
Location: | 1580387 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g618750 | FAP246 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGGCTCGCCGCACTGGTGCGTGTGTGCGT |
Internal bar code: | ATCTCCAGCCCTGCGTAGACGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 290 |
LEAP-Seq percent confirming: | 46.2704 |
LEAP-Seq n confirming: | 7574 |
LEAP-Seq n nonconfirming: | 8795 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTTGACTCGCTCAATGCAC |
Suggested primer 2: | CATTCGGATGAGTGTGATGC |