| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156564 |
| Chromosome: | chromosome 12 |
| Location: | 816733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGGGTCCGGACAAAGTAGGCAGCCGG |
| Internal bar code: | AGGGCTCACCTCGGAGCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 94.7137 |
| LEAP-Seq n confirming: | 430 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCCTGGTTTCTTTGGGA |
| Suggested primer 2: | TGGATTTCCATTTCCACCAT |