Insertion junction: LMJ.RY0402.156569_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.156569
Chromosome:chromosome 1
Location:4300040
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g028950 (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAATGGGTCAATCCAAGTCACTGAGGG
Internal bar code:GCGCTAAACCTAATGAACTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1011
LEAP-Seq percent confirming:98.8235
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACAAGCTTCCCACCAC
Suggested primer 2:GGTTGACAGGGCGTAAGGTA