Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.156569 |
Chromosome: | chromosome 1 |
Location: | 4300040 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g028950 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAATGGGTCAATCCAAGTCACTGAGGG |
Internal bar code: | GCGCTAAACCTAATGAACTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1011 |
LEAP-Seq percent confirming: | 98.8235 |
LEAP-Seq n confirming: | 84 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACAACAAGCTTCCCACCAC |
Suggested primer 2: | GGTTGACAGGGCGTAAGGTA |