| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156577 |
| Chromosome: | chromosome 16 |
| Location: | 7066468 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679109 | YEE3 | Integral membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAAGCCAAGTAGCCATGATTCCCATTC |
| Internal bar code: | AGGATTGATAGTCTAGCCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.5955 |
| LEAP-Seq n confirming: | 1231 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGATGACACACCAGACC |
| Suggested primer 2: | CACGATACGACATGACGAGG |