| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156585 |
| Chromosome: | chromosome 6 |
| Location: | 1065281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256500 | (1 of 2) PTHR14187//PTHR14187:SF5 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTGCGACTGCGGCCGCCTCCCGCGGAC |
| Internal bar code: | CGCGATGACCGGCTGCTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.5498 |
| LEAP-Seq n confirming: | 1769 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCGAAGATTCAGATGG |
| Suggested primer 2: | AACGGTTGAGGAGTTGGTTG |