| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156586 |
| Chromosome: | chromosome 12 |
| Location: | 7361848 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557000 | ATG12,APG12 | Autophagy-related protein; (1 of 1) K08336 - ubiquitin-like protein ATG12 (ATG12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGCGGTGCCGGTATGGTCATGCGTGG |
| Internal bar code: | CCAATCGGGCCAAACGGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 26.5916 |
| LEAP-Seq n confirming: | 16678 |
| LEAP-Seq n nonconfirming: | 46041 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAGGTGAACTACGCCCC |
| Suggested primer 2: | TGTGCAAGAATGAAGCCAAG |