| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156599 |
| Chromosome: | chromosome 13 |
| Location: | 451537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564812 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATGATTATGTACGCGCAAAGCCCTAGC |
| Internal bar code: | CGAAATGCGTCCCTGCGTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1109 |
| LEAP-Seq percent confirming: | 98.3223 |
| LEAP-Seq n confirming: | 1641 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTTGATGGGATCTCTG |
| Suggested primer 2: | TTCTCAACAACACCAGCAGC |