Insertion junction: LMJ.RY0402.156645_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156645
Chromosome:chromosome 2
Location:285302
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCTGCTTACAATGGAGAAGGAGGAAGTG
Internal bar code:TGCGGGACGGTGCTTTGTCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:351
LEAP-Seq percent confirming:99.5181
LEAP-Seq n confirming:413
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTACCCTGATGTCGAGTT
Suggested primer 2:ACGCGCTTGATATTCAGCTT