| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156660 |
| Chromosome: | chromosome 13 |
| Location: | 574736 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565450 | PURB,PURB1 | Adenylosuccinate lyase; (1 of 1) K01756 - adenylosuccinate lyase (purB, ADSL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCATGCGGCAAGCACCAAAAGTGCGT |
| Internal bar code: | TACGGTAAAAGCCACTTAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGCTTATGCTTGAAGG |
| Suggested primer 2: | GGTGGGGGTATCAATGACTG |