| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156661 |
| Chromosome: | chromosome 1 |
| Location: | 2678621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015750 | (1 of 2) PF07978 - NIPSNAP (NIPSNAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGGGTCGGGGGCACTAAACGGGGTGTT |
| Internal bar code: | GCCTTTTCGTCGTAGAGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1108 |
| LEAP-Seq percent confirming: | 98.7299 |
| LEAP-Seq n confirming: | 17412 |
| LEAP-Seq n nonconfirming: | 224 |
| LEAP-Seq n unique pos: | 129 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCAGACGAAAACCTTTG |
| Suggested primer 2: | CCAACAGAGGAGCACACAGA |