Insertion junction: LMJ.RY0402.156666_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.156666
Chromosome:chromosome_7
Location:941979
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g319150 PTP4 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTAAGAACACGCACCAAAACACATCTGTCT
Internal bar code:GTCTGGCTCAGTGTTCTCTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:255
LEAP-Seq percent confirming:99.5689
LEAP-Seq n confirming:8083
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGTGTGTGTGGGGTTA
Suggested primer 2:TCGATCAGGTAGCAGCACAC