Insertion junction: LMJ.RY0402.156669_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156669
Chromosome:chromosome 14
Location:2746905
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g627050 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGTGGCATGTGGTTGTACGAGCGGGCTA
Internal bar code:GACTCCCCGCGTTATTCGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1137
LEAP-Seq percent confirming:99.0389
LEAP-Seq n confirming:2061
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACTCCCTCTCACCC
Suggested primer 2:CAATTGACTTTGCCTGCTGA