| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156670 |
| Chromosome: | chromosome 1 |
| Location: | 4483509 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030550 | UBX1 | (1 of 1) PTHR13020:SF34 - UBX DOMAIN-CONTAINING PROTEIN 4; possible ubiquitin-proteasome pathway protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGCACCAGGCTGGGCCCCATCGAGGC |
| Internal bar code: | GCTACATCGTTGAGTGTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 99.3186 |
| LEAP-Seq n confirming: | 583 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCTCTCTTGCGATACG |
| Suggested primer 2: | TAGCAGCTCCCCTACCAAGA |