| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156673 |
| Chromosome: | chromosome 16 |
| Location: | 2897931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663750 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACTGTTGACTACAATCCCCCTGCCCC |
| Internal bar code: | CGTTCCTCACGTTTTTATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 99.7381 |
| LEAP-Seq n confirming: | 3047 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACGACCCTATCATGC |
| Suggested primer 2: | GCCAAGCAGAATACTCCCAG |