| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156680 |
| Chromosome: | chromosome 9 |
| Location: | 2840583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388700 | (1 of 2) PTHR23257//PTHR23257:SF428 - SERINE-THREONINE PROTEIN KINASE // INACTIVE SERINE/THREONINE-PROTEIN KINASE DDB_G0280131-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAGGCTCTGTAGGCGGGCACGTGACGT |
| Internal bar code: | GAACAGGGTCATCACCAATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.8315 |
| LEAP-Seq n confirming: | 2370 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGCATGTGCTGATCTAA |
| Suggested primer 2: | GCGGCCTCAGACTAGATACG |