Insertion junction: LMJ.RY0402.156687_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156687
Chromosome:chromosome 1
Location:2963426
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g018100 (1 of 1) 3.1.3.16//3.1.3.27//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Phosphatidylglycerophosphatase // Protein-tyrosine-phosphatase / PTPase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACCCCAACAATACTACACGAGGTTTGGT
Internal bar code:TAGGAAGGCGGCTCTCAGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:798
LEAP-Seq percent confirming:98.9349
LEAP-Seq n confirming:1672
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTCGAGACCTTCTCAGG
Suggested primer 2:ATAATCACCCAGCACCAACC