| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156697 |
| Chromosome: | chromosome 1 |
| Location: | 3675006 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023650 | Probable amino acid/metabolite permease; (1 of 4) PTHR11785:SF367 - AMINO-ACID PERMEASE BAT1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCAGGTTGGCGCGAGGGCCTGCGGGC |
| Internal bar code: | TTCACAGAGCATGCGGCTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 66.4525 |
| LEAP-Seq n confirming: | 17481 |
| LEAP-Seq n nonconfirming: | 8825 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCATGCACAGCAGTCT |
| Suggested primer 2: | TGGCTACAACTTCGCTTCCT |