| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156707 |
| Chromosome: | chromosome 1 |
| Location: | 1543120 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008000 | (1 of 1) K18666 - activating signal cointegrator complex subunit 1 (ASCC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGTGGCAATCCCTCCAAAGGCCCCAAA |
| Internal bar code: | ACACTGGACGCAGGGCAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 94.9126 |
| LEAP-Seq n confirming: | 1194 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAAAACCAGACACCCT |
| Suggested primer 2: | CCCTTTCACACACTCCAGGT |